使用敲除细胞株进行验证RabMAb

Anti-CSK抗体[EPR6771] (ab125005)

概述

  • 产品名称Anti-CSK抗体[EPR6771]
    参阅全部 CSK 一抗
  • 描述
    兔单克隆抗体[EPR6771] to CSK
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human CSK.

  • 阳性对照
    • Jurkat, Ramos and RAW 264.7 cell lysates; Human tonsil tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab125005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
    • 组织特异性Expressed in lung and macrophages.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • 翻译后修饰Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
    • 细胞定位Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C SRC antibody
      • C SRC kinase antibody
      • C src Tyrosine Kinase antibody
      • C-SRC kinase antibody
      • c-src tyrosine kinase antibody
      • Csk A antibody
      • CSK antibody
      • CSK_HUMAN antibody
      • CYTOPLASMIC TYROSINE KINASE antibody
      • EC 2.7.10.2 antibody
      • MGC112926 antibody
      • MGC117393 antibody
      • MGC154049 antibody
      • P60 Src antibody
      • Protein tyrosine kinase CYL antibody
      • Protein-tyrosine kinase CYL antibody
      • Proto oncogene tyrosine protein kinase antibody
      • Tyrosine protein kinase CSK antibody
      • Tyrosine-protein kinase CSK antibody
      • zgc:154049 antibody
      see all

    Anti-CSK antibody [EPR6771] 图像



    • Predicted band size : 50 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CSK knockout HAP1 cell lysate (20 µg)
      Lane 3: Ramos cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab125005 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab125005 was shown to specifically react with CSK when CSK knockout samples were used. Wild-type and CSK knockout samples were subjected to SDS-PAGE. ab125005 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-CSK antibody [EPR6771] (ab125005) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : RAW 264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 50 kDa
    • ab125005 at 1/50 dilution staining CSK in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-CSK antibody [EPR6771] (ab125005)参考文献

    ab125005 has not yet been referenced specifically in any publications.

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