Anti-CSK抗体(ab744)
Key features and details
- Goat polyclonal to CSK
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-CSK抗体
参阅全部 CSK 一抗 -
描述
山羊多克隆抗体to CSK -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Dog -
免疫原
Synthetic peptide corresponding to Human CSK aa 400 to the C-terminus (C terminal).
Database link: NP_004374.1 -
阳性对照
- Jurkat cell lysate, Human colon tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use at an assay dependent concentration.
|
说明 |
---|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use at an assay dependent concentration. |
靶标
-
功能
Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases. -
组织特异性
Expressed in lung and macrophages. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰
Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro. -
细胞定位
Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts. - Information by UniProt
-
数据库链接
- Entrez Gene: 509246 Cow
- Entrez Gene: 487649 Dog
- Entrez Gene: 1445 Human
- Entrez Gene: 12988 Mouse
- Omim: 124095 Human
- SwissProt: Q0VBZ0 Cow
- SwissProt: P41240 Human
- SwissProt: P41241 Mouse
see all -
别名
- C SRC antibody
- C SRC kinase antibody
- C src Tyrosine Kinase antibody
see all
图片
-
ab744 staining (0.5
µ g/ml) of Jurkat lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
ab744 at 4µg/ml staining CSK in Human Colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
-
Anti-CSK antibody (ab744) at 1 µg/ml + Mouse spleen lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
数据表及文件
-
Datasheet download
文献 (2)
ab744 被引用在 2 文献中.
- Yeh WL et al. Fulvestrant-induced cell death and proteasomal degradation of estrogen receptor a protein in MCF-7 cells require the CSK c-Src tyrosine kinase. PLoS One 8:e60889 (2013). WB ; Human . PubMed: 23593342
- Ribeiro-Oliveira A et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB ; Human . PubMed: 18701577