DREAM overexpression 293T裂解物(whole cell) (ab94275)
概述
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产品名称
DREAM overexpression 293T裂解物(whole cell) -
常规说明
ab94275 is a 293T cell transfected lysate in which Human DREAM has been transiently over-expressed using a pCMV-DREAM plasmid. The lysate is provided in 1X Sample Buffer.
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception. Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation. PTM: Palmitoylated. Palmitoylation enhances association with the plasma membrane. Proteolytically cleaved by caspase-3. Phosphorylation at Ser-63 inhibits cleavage by CASP3. Similarity: Belongs to the recoverin family. Contains 4 EF-hand domains. Tissue specificity: Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94275于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94275 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-DREAM antibody (ab57203) at 1/500 dilution
Lane 1 :DREAM overexpression 293T lysate (whole cell) (ab94275)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94275 尚未被引用在任何文献中。