CSEN overexpression 293T裂解液(全细胞) (ab94275)

概述

  • 产品名称CSEN overexpression 293T裂解液(全细胞)
  • 描述
    CSEN overexpression裂解液
  • 常规说明ab94275 is a 293T cell transfected lysate in which Human CSEN has been transiently over-expressed using a pCMV-CSEN plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception. Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation. PTM: Palmitoylated. Palmitoylation enhances association with the plasma membrane. Proteolytically cleaved by caspase-3. Phosphorylation at Ser-63 inhibits cleavage by CASP3. Similarity: Belongs to the recoverin family. Contains 4 EF-hand domains. Tissue specificity: Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.

应用

Our Abpromise guarantee covers the use of ab94275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

CSEN overexpression 293T lysate (whole cell) 图像

  • ab94275 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-CSEN antibody (ab57203) at 1/500 dilution

    Lane 1 : CSEN overexpression 293T lysate (whole cell) (ab94275)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

CSEN overexpression 293T lysate (whole cell) (ab94275)参考文献

ab94275 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94275.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"