Anti-CSDE1/NRU抗体(ab96124)
Key features and details
- Rabbit polyclonal to CSDE1/NRU
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CSDE1/NRU抗体
参阅全部 CSDE1/NRU 一抗 -
描述
兔多克隆抗体to CSDE1/NRU -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to Human CSDE1/NRU aa 167-372. (AAH32446)
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阳性对照
- HeLa whole cell lysate (ab150035); A549, HepG2 and HCT116 lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96124于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 88 kDa.
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IHC-P | (1) |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 88 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. -
序列相似性
Contains 9 CSD (cold-shock) domains. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 513993 Cow
- Entrez Gene: 7812 Human
- Entrez Gene: 229663 Mouse
- Entrez Gene: 117180 Rat
- Omim: 191510 Human
- SwissProt: O75534 Human
- SwissProt: Q91W50 Mouse
- SwissProt: P18395 Rat
see all -
别名
- Cold shock domain containing E1 RNA binding antibody
- Cold shock domain containing protein E1 antibody
- Cold shock domain-containing protein E1 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ab96124 at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min -
All lanes : Anti-CSDE1/NRU antibody (ab96124) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : A375 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : The HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 88 kDa -
Immunofluorescence analysis of methanol-fixed HeLa, using ab96124 antibody at 1:200 dilution. Costained with Hoechst 33342.
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Anti-CSDE1/NRU antibody (ab96124) at 1/3000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 88 kDa
7.5% SDS PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab96124 被引用在 3 文献中.
- Martinez-Useros J et al. UNR/CSDE1 Expression Is Critical to Maintain Invasive Phenotype of Colorectal Cancer through Regulation of c-MYC and Epithelial-to-Mesenchymal Transition. J Clin Med 8:N/A (2019). PubMed: 31027221
- Martinez-Useros J et al. UNR/CDSE1 expression as prognosis biomarker in resectable pancreatic ductal adenocarcinoma patients: A proof-of-concept. PLoS One 12:e0182044 (2017). PubMed: 28763470
- Ju Lee H et al. A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. Nat Commun 8:1456 (2017). PubMed: 29129916