概述

  • 产品名称
  • 描述
    兔多克隆抗体to CSAD
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human CSAD.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Mouse Liver Tissue Lysate; Mouse Kidney Tissue Lysate; Rat Liver Tissue Lysate; Rat Kidney Tissue Lysate ; NIH 3T3 Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab101847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

靶标

图片

  • All lanes : Anti-CSAD antibody (ab101847) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Kidney (Rat) Tissue Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 100 kDa,130 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

实验方案

文献

This product has been referenced in:
  • Terrill JR  et al. Levels of inflammation and oxidative stress, and a role for taurine in dystropathology of the Golden Retriever Muscular Dystrophy dog model for Duchenne Muscular Dystrophy. Redox Biol 9:276-286 (2016). WB . Read more (PubMed: 27611888) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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