The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. For 2 hours.
Use at an assay dependent dilution.
Use at an assay dependent concentration.
Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding.
Only very low amounts in the lens.
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
Immunocytochemistry analysis using ab110310 at 2µg/ml staining CRYZ in HELa cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green).
Using MitoSciences' standard immunoprecipitation protocol, ab110310 precipitates the 35kDa CRYZ protein from human liver and HepG2 lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with silver nitrate.