Anti-CRYBA4抗体(ab97771)
Key features and details
- Rabbit polyclonal to CRYBA4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CRYBA4抗体 -
描述
兔多克隆抗体to CRYBA4 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Guinea pig, Cow, Dog, Pig, Monkey -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 1 - 150 of Human CRYBA4 (NP_001877).
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阳性对照
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lyates IF: HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97771于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 22 kDa.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 22 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Crystallins are the dominant structural components of the vertebrate eye lens. -
疾病相关
Defects in CRYBA4 are the cause of cataract zonular type 2 (CZ2) [MIM:610425]; also known as lamellar cataract 2. A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes.
Defects in CRYBA4 are a cause of microphthalmia isolated with cataract type 4 (MCOPCT4) [MIM:610426]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present. -
序列相似性
Belongs to the beta/gamma-crystallin family.
Contains 4 beta/gamma crystallin 'Greek key' domains. -
结构域
Has a two-domain beta-structure, folded into four very similar Greek key motifs. - Information by UniProt
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数据库链接
- Entrez Gene: 282204 Cow
- Entrez Gene: 486334 Dog
- Entrez Gene: 100306973 Guinea pig
- Entrez Gene: 1413 Human
- Entrez Gene: 12959 Mouse
- Entrez Gene: 64348 Rat
- Omim: 123631 Human
- SwissProt: P11842 Cow
see all -
别名
- Beta A4 crystallin antibody
- Beta crystallin A4 antibody
- Beta-A4 crystallin antibody
see all
图片
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All lanes : Anti-CRYBA4 antibody (ab97771) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
12% SDS PAGE -
ab97771, at a 1/200 dilution, staining CRYBA4 in paraformaldehyde-fixed HeLa by Immunofluorescence analysis. Right image is merged with a DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab97771 尚未被引用在任何文献中。