概述

  • 产品名称Anti-CRY2抗体
    参阅全部 CRY2 一抗
  • 描述
    兔多克隆抗体to CRY2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 543-593 of Human CRY2 (AAH41814.1)

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells

性能

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应用

Our Abpromise guarantee covers the use of ab93802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 67 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK
      NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK
      NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.
    • 组织特异性Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle.
    • 序列相似性Belongs to the DNA photolyase class-1 family.
      Contains 1 DNA photolyase domain.
    • 翻译后修饰Phosphorylation on Ser-266 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSKNE requires interaction with PER1 or PER2.
      Ubiquitinated by the SCF(FBXL3) and SCF(FBXL21) complex, leading to its degradation.
    • 细胞定位Cytoplasm. Nucleus. Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.
    • Information by UniProt
    • 数据库链接
    • 别名
      • cry2 antibody
      • CRY2_HUMAN antibody
      • cryptochrome 2 (photolyase like) antibody
      • Cryptochrome 2 antibody
      • Cryptochrome-2 antibody
      • FLJ10332 antibody
      • growth inhibiting protein 37 antibody
      • HCRY2 antibody
      • KIAA0658 antibody
      • PHLL2 antibody
      • Photolyase like antibody
      see all

    Anti-CRY2 antibody 图像

    • All lanes : Anti-CRY2 antibody (ab93802) at 0.04 µg/ml

      Lane 1 : whole HeLa cell lysate at 50 µg
      Lane 2 : whole HeLa cell lysate at 15 µg
      Lane 3 : whole HeLa cell lysate at 5 µg
      Lane 4 : whole 293T cell lysate at 50 µg


      Predicted band size : 67 kDa
    • Immunoprecipitation analysis of CRY2 expression in whole HeLa cells lysate (1 mg for IP, 20% of IP loaded) using ab93802 at 3 ug/mg lysate (Left Lane). Right lane represent IP antibody control. Subsequent WB analysis was performed using 1ug/ml ab93802.

    Anti-CRY2 antibody (ab93802)参考文献

    ab93802 has not yet been referenced specifically in any publications.

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