Anti-CRY2抗体(ab93802)
Key features and details
- Rabbit polyclonal to CRY2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CRY2抗体
参阅全部 CRY2 一抗 -
描述
兔多克隆抗体to CRY2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 543-593 of Human CRY2 (AAH41814.1)
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阳性对照
- Whole cell lysate from HeLa and 293T cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93802于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 67 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 67 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK
NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK
NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. -
组织特异性
Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle. -
序列相似性
Belongs to the DNA photolyase class-1 family.
Contains 1 DNA photolyase domain. -
翻译后修饰
Phosphorylation on Ser-266 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSKNE requires interaction with PER1 or PER2.
Ubiquitinated by the SCF(FBXL3) and SCF(FBXL21) complex, leading to its degradation. -
细胞定位
Cytoplasm. Nucleus. Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL. - Information by UniProt
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数据库链接
- Entrez Gene: 1408 Human
- Omim: 603732 Human
- SwissProt: Q49AN0 Human
- Unigene: 532491 Human
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别名
- cry2 antibody
- CRY2_HUMAN antibody
- cryptochrome 2 (photolyase like) antibody
see all
图片
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All lanes : Anti-CRY2 antibody (ab93802) at 0.04 µg/ml
Lane 1 : whole HeLa cell lysate at 50 µg
Lane 2 : whole HeLa cell lysate at 15 µg
Lane 3 : whole HeLa cell lysate at 5 µg
Lane 4 : whole 293T cell lysate at 50 µg
Predicted band size: 67 kDa -
Immunoprecipitation analysis of CRY2 expression in whole HeLa cells lysate (1 mg for IP, 20% of IP loaded) using ab93802 at 3 ug/mg lysate (Left Lane). Right lane represent IP antibody control. Subsequent WB analysis was performed using 1ug/ml ab93802.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab93802 被引用在 1 文献中.
- Gorelik M et al. A Structure-Based Strategy for Engineering Selective Ubiquitin Variant Inhibitors of Skp1-Cul1-F-Box Ubiquitin Ligases. Structure 26:1226-1236.e3 (2018). PubMed: 30033217