重组
RabMAb

Anti-CRTC3抗体[EPR3440] (ab91654)

概述

  • 产品名称
    Anti-CRTC3抗体[EPR3440]
    参阅全部 CRTC3 一抗
  • 描述
    兔单克隆抗体[EPR3440] to CRTC3
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CRTC3. The exact sequence is proprietary.

  • 阳性对照
    • 293T and Jurkat cell lysates and Human tonsil tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab91654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/20000. Predicted molecular weight: 67 kDa.

For unpurified, use 1/1000 - 10000.

IHC-P 1/100 - 1/250.
ICC/IF 1/100. PubMed: 23033494
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
    • 组织特异性
      Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach.
    • 序列相似性
      Belongs to the TORC family.
    • 细胞定位
      Nucleus. Cytoplasm. Appears to be mainly nuclear.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CREB regulated transcription coactivator 3 antibody
      • CREB-regulated transcription coactivator 3 antibody
      • CRTC 3 antibody
      • CRTC3 antibody
      • CRTC3_HUMAN antibody
      • FLJ21868 antibody
      • TORC 3 antibody
      • TORC-3 antibody
      • TORC3 antibody
      • Transducer of CREB protein 3 antibody
      • Transducer of regulated cAMP response element binding protein (CREB) 3 antibody
      • Transducer of regulated cAMP response element binding protein 3 antibody
      • Transducer of regulated cAMP response element-binding protein 3 antibody
      • Transducer of regulated CREB protein 3 antibody
      see all

    Anti-CRTC3 antibody [EPR3440] 图像

    • Immunofluorescence staining of Jurkat cells with purified ab91654 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab91654 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
    • ChIP analysis using ab91654 binding CRTC3 in Human neuroblastoma cells. Cells were cross-linked for 10 minutes with 1% formaldehyde. Samples were incubated with primary antibody (0.2µg/10^6 cells) for 12 hours at 4°C. Protein binding was detected using real-time PCR.
      Positive control: GAPDH.
      Negative Control: Primers for region not expressed.

       

      See Abreview

    • Unpurified ab91654 at 1/100 dilution staining CRTC3 in Human tonsil by immunohistochemistry (paraffin-embedded section).

    • All lanes : Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/20000 dilution (purified)

      Lane 1 : Raw 264.7 whole cell lysate
      Lane 2 : PC-12 whole cell lysate
      Lane 3 : NIH/3T3 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 67 kDa
      Observed band size : 76 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/20000 dilution (purified)

      Lane 1 : HEK293 whole cell lysate
      Lane 2 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 67 kDa
      Observed band size : 76 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded rat spleen with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemical staining of paraffin embedded mouse spleen with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemical staining of paraffin embedded human tonsil with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • All lanes : Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 67 kDa

    Anti-CRTC3 antibody [EPR3440] (ab91654)参考文献

    This product has been referenced in:
    • Jurek B  et al. Oxytocin Regulates Stress-Induced Crf Gene Transcription through CREB-Regulated Transcription Coactivator 3. J Neurosci 35:12248-60 (2015). WB, ChIP ; Human . Read more (PubMed: 26338335) »
    • Patel K  et al. The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver. Nat Commun 5:4535 (2014). WB . Read more (PubMed: 25088745) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SKOv3 ovarian cancer cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% BIS-TRIS)
    Loading amount
    20 µg
    Specification
    SKOv3 ovarian cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 21 2017

    ChIP

    Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Human Cell lysate - nuclear (neuroblastoma cells)
    Specification
    neuroblastoma cells
    Negative control
    negative control primers for a region surely not to be expressed. The primers were bought from SABioscience (Quiagen) and are named: negative ChIP control primer #1.
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde 1%
    Positive control
    GAPDH
    Username

    Dr. Ben Jurek

    Verified customer

    提交于 May 20 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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