Anti-CRSP9抗体(ab50687)

概述

  • 产品名称Anti-CRSP9抗体
    参阅全部 CRSP9 一抗
  • 描述
    兔多克隆抗体to CRSP9
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab50687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Peptide ELISA titre: 1/312500.

WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 序列相似性Belongs to the Mediator complex subunit 7 family.
  • 翻译后修饰Constitutively sumoylated.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activator recruited cofactor 34 kDa component antibody
    • Activator-recruited cofactor 34 kDa component antibody
    • ARC34 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 9 antibody
    • Cofactor required for Sp1 transcriptional activation, subunit 9 (33kD) antibody
    • Cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa antibody
    • CRSP complex subunit 9 antibody
    • CRSP33 antibody
    • CRSP9 antibody
    • hMED7 antibody
    • MED7 antibody
    • MED7_HUMAN antibody
    • Mediator complex subunit 7 antibody
    • Mediator of RNA polymerase II transcription subunit 7 antibody
    • MGC12284 antibody
    • RNA polymerase transcriptional regulation mediator subunit 7 homolog antibody
    • Transcriptional coactivator CRSP33 antibody
    see all

Anti-CRSP9 antibody 图像

  • Anti-CRSP9 antibody (ab50687) at 0.25 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution.

    Predicted band size : 27 kDa
    Observed band size : 27 kDa

Anti-CRSP9 antibody (ab50687)参考文献

ab50687 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (293)
Loading amount 10 µg
Specification 293
Gel Running Conditions Reduced Denaturing (16%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Apr 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"