概述

  • 产品名称Anti-CRSP8抗体
    参阅全部 CRSP8 一抗
  • 描述
    兔多克隆抗体to CRSP8
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • 免疫原

    A region within synthetic peptide: VRSFMTWLRS YIKLFQAPCQ RCGKFLQDGL PPTWRDFRTL EAFHDTCRQ, corresponding to C terminal amino acids 263-311 of Human CRSP8

  • 阳性对照
    • HepG2 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab66370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

  • 功能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 序列相似性Belongs to the Mediator complex subunit 27 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cofactor required for Sp1 transcriptional activation subunit 8 (34kD) antibody
    • Cofactor required for Sp1 transcriptional activation subunit 8 34kDa antibody
    • Cofactor required for Sp1 transcriptional activation subunit 8 antibody
    • CRAP 34 antibody
    • CRAP34 antibody
    • CRSP 34 antibody
    • CRSP 8 antibody
    • CRSP complex subunit 8 antibody
    • CRSP34 antibody
    • CRSP8 antibody
    • MED 27 antibody
    • Med27 antibody
    • MED27_HUMAN antibody
    • Mediator complex subunit 27 antibody
    • Mediator of RNA polymerase II transcription subunit 27 antibody
    • MGC11274 antibody
    • P37 TRAP/SMCC/PC2 subunit antibody
    • Transcriptional coactivator CRSP34 antibody
    • TRAP 37 antibody
    • TRAP37 antibody
    see all

Anti-CRSP8 antibody 图像

  • Anti-CRSP8 antibody (ab66370) at 5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

Anti-CRSP8 antibody (ab66370)参考文献

ab66370 has not yet been referenced specifically in any publications.

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