The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 5-10 µg/mg of lysate.
Is unsuitable for WB.
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Belongs to the Mediator complex subunit 26 family. Contains 1 TFIIS N-terminal domain.
Detection of CRSP7 by Western Blot of Immunprecipitate.
An antibody to CRSP7, at 0.4 µg/ml, staining CRSP7 in HeLa whole cell lysate immunoprecipitated using ab114092 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 15 seconds.