Cross-linked Allophycocyanin activated with SMCC (ab123481)

概述

描述

  • 性质Native
  • 来源Native
  • 氨基酸序列
    • 额外的序列信息purified from natural source, either algae or cyanobacteria.

技术指标

Our Abpromise guarantee covers the use of ab123481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Flow Cytometry

    Immunocytochemistry/ Immunofluorescence

  • 形式Liquid
  • 补充说明

    SMCC Activated chemically cross-linked Allophycocyanin (CL-APC), with a maleimide group introduced, allows it to be conveniently conjugated to the thiol groups of proteins, without the need for additional activation. Abs/Em = 651/662 nm

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.

    pH: 6.00
    Constituents: 99% MES, EDTA
    Note: Contains perservative.

常规信息

  • 别名
    • apcB
    • Allophycocyanin alpha chain
    • Allophycocyanin beta chain
    • apcA
  • 相关性Allophycocyanin is a protein from the light-harvesting phycobiliprotein family, along with phycocyanin, phycoerythrin and phycoerythrocyanin. It is an accessory pigment to chlorophyll. All phycobiliproteins are water soluble and therefore cannot exist within the membrane like carotenoids, but aggregate to form clusters that adhere to the membrane called phycobilisomes. Allophycocyanin absorbs and emits red light (650 & 660 nm max, respectively), is readily found in Cyanobacteria and red algae, and is a useful immunoassay tool. APC is made up of alpha and beta subunits and is present as a trimer (alpha beta)3. The trimer is unstable and susceptible to dissociation at low concentrations. The monomer, alpha beta, has a lower fluorescence quantum yield compared to the trimer and the maximal absorption is shifted to 620 nm. The chemically crosslinked APC (CL-APC) trimer is much more stable than the native protein, but still retains comparable spectroscopic properties. The extinction coefficient of CL-APC at 650 nm is 700,000 cm-1M-1.

Cross-linked Allophycocyanin activated with SMCC (ab123481)参考文献

ab123481 has not yet been referenced specifically in any publications.

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