The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: Use at 2 - 5 µg/mg of lysate.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing.
组织特异性Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung.
序列相似性Belongs to the Luc7 family.
翻译后修饰Phosphorylated in vitro by SRPK1, SRPK2 and CLK1.
细胞定位Nucleus speckle. The subnuclear localization is affected by cisplatin.
Detection of human CROP in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80415 at 3 µg/mg lysate for IP (lane 1). Lane 2 represents control IgG IP. Subsequent WB detection used ab80412 (which recognizes an upstream epitope) at 1 µg/ml.
Anti-CROP antibody (ab80415)参考文献
has not yet been referenced specifically in any publications.