The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
应用说明Is unsuitable for WB.
功能Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing.
组织特异性Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung.
序列相似性Belongs to the Luc7 family.
翻译后修饰Phosphorylated in vitro by SRPK1, SRPK2 and CLK1.
细胞定位Nucleus speckle. The subnuclear localization is affected by cisplatin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse colon carcinoma (right) tissue labelling CROP with ab80412 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - CROP antibody (ab80412)
Detection of Human CROP in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80412 at 3 µg/mg lysate for IP (lane 1). ab80412 was used at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
Anti-CROP antibody (ab80412)参考文献
has not yet been referenced specifically in any publications.