Anti-CROP抗体(ab79231)
Key features and details
- Rabbit polyclonal to CROP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CROP抗体
参阅全部 CROP 一抗 -
描述
兔多克隆抗体to CROP -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chicken, Cow, Orangutan -
免疫原
Synthetic peptide corresponding to Human CROP aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab87825) -
阳性对照
- This antibody gave a positive signal in the following whole cell lysates: HeLa; U2OS; Jurkat; HEK293; HepG2; Caco2.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79231于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing. -
组织特异性
Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung. -
序列相似性
Belongs to the Luc7 family. -
翻译后修饰
Phosphorylated in vitro by SRPK1, SRPK2 and CLK1. -
细胞定位
Nucleus speckle. The subnuclear localization is affected by cisplatin. - Information by UniProt
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数据库链接
- Entrez Gene: 539955 Cow
- Entrez Gene: 51747 Human
- Entrez Gene: 67684 Mouse
- Entrez Gene: 100172991 Orangutan
- Omim: 609434 Human
- SwissProt: Q3SX41 Cow
- SwissProt: O95232 Human
- SwissProt: Q5SUF2 Mouse
see all -
别名
- cAMP regulatory element-associated protein 1 antibody
- Cisplatin resistance-associated overexpressed protein antibody
- Cisplatin resistance-associated-overexpressed protein antibody
see all
图片
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All lanes : Anti-CROP antibody (ab79231) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
The 60 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to CROP. -
ICC/IF image of ab79231 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79231, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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