概述

  • 产品名称
  • 描述
    兔多克隆抗体to CROCC
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: VKRLEKQNLE KDQVNKDLTE KLEALESLRL QEQAALETED GEGLQQTLRD LAQAVLSDSE SGVQLSGSER TADASNGS, corresponding to amino acids 406-483 of Human CROCC

  • 阳性对照
    • Human fallopian tube tissue; U2OS cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

靶标

  • 功能
    Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis.
  • 序列相似性
    Belongs to the rootletin family.
  • 翻译后修饰
    Phosphorylated by NEK2 which may regulate its association with centrosomes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cytoplasm > cytoskeleton > centrosome > centriole. In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ciliary rootlet coiled coil antibody
    • Ciliary rootlet coiled-coil protein antibody
    • Crocc antibody
    • CROCC_HUMAN antibody
    • KIAA0445 antibody
    • Rootletin antibody
    see all

图片

  • ab121653, at 1/150 dilution, staining CROCC in Human fallopian tube tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • ab121653 at 4 µg/ml staining CROCC in PFA/Triton X-100 fixed/permeabilized U2OS cells by Immunofluorescence (green).

文献

ab121653 has not yet been referenced specifically in any publications.

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