Anti-CRNKL1抗体[2212C1a] (ab51348)

概述

  • 产品名称Anti-CRNKL1抗体[2212C1a]
    参阅全部 CRNKL1 一抗
  • 描述
    小鼠单克隆抗体[2212C1a] to CRNKL1
  • 经测试应用适用于: ICC/IF, WB, Dot blotmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant human CRNKL1 fragment

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4).
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Ab51348 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
  • 克隆单克隆
  • 克隆编号2212C1a
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50.
WB Use at an assay dependent dilution. Predicted molecular weight: 100 kDa.
Dot blot Use at an assay dependent dilution.

靶标

  • 功能Involved in pre-mRNA splicing process.
  • 组织特异性Widely expressed. Highly expressed in testis. Not expressed in brain and lung.
  • 序列相似性Belongs to the crooked-neck family.
    Contains 17 HAT repeats.
  • 细胞定位Nucleus speckle. Cytoplasm. Colocalizes with core spliceosomal snRNP proteins. Also diffusely expressed in cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1200013P10Rik antibody
    • 5730590A01Rik antibody
    • C80326 antibody
    • CLF antibody
    • Clf1 antibody
    • CRN antibody
    • Crn crooked neck like 1 (Drosophila) antibody
    • Crnkl1 antibody
    • CRNL1_HUMAN antibody
    • Crooked neck homolog antibody
    • Crooked neck like protein 1 antibody
    • Crooked neck pre mRNA splicing factor 1 antibody
    • Crooked neck-like protein 1 antibody
    • hCrn antibody
    • MGC55327 antibody
    • MGC93283 antibody
    • MSTP021 antibody
    • SYF3 antibody
    • SYF3 pre mRNA splicing factor antibody
    see all

Anti-CRNKL1 antibody [2212C1a] 图像

  • Anti-CRNKL1 antibody [2212C1a] (ab51348) + Recombinant CRNKL1

    Predicted band size : 100 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab51348 staining CRNKL1 in Human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 01.% Triton X100 in PBS prior to blocking in 0.5% BSA for 20 minutes at room temperature. The primary antibody was diluted 1/50 in 0.5% BSA in PBS and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Mouse polyclonal, diluted 1/400. Nuclei were counterstained with Hoechst (blue)

    See Abreview

Anti-CRNKL1 antibody [2212C1a] (ab51348)参考文献

ab51348 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (glioma cell line C6 (rat))
Specification glioma cell line C6 (rat)
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line D54MG)
Specification human glioblastoma cell line D54MG
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell lines)
Specification human glioblastoma cell lines
Fixative Paraformaldehyde
Permeabilization Yes - Tritonx100 0.1% in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"