概述

  • 产品名称Anti-CRMP5抗体
    参阅全部 CRMP5 一抗
  • 描述
    小鼠单克隆抗体to CRMP5
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment: SFPDTVYKKL VQREKTLKVR GVDRTPYLGD VAVVVHPGKK EMGTPLADTP TRPVTRHGGM RDLHESSFSL SGSQIDDHVP KRASARILAP PGGRSSGIW, corresponding to amino acids 466-564 of human CRMP5 (NP_064519) with a 26 kDa tag.

  • 阳性对照
    • CRMP5 transfected 293T cell lysate; recombinant tagged human CRMP5 fragment.

性能

应用

Our Abpromise guarantee covers the use of ab77158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.
ELISA Use at an assay dependent dilution.

靶标

Anti-CRMP5 antibody 图像

  • Anti-CRMP5 antibody (ab77158) at 5 µg/ml + recombinant tagged human CRMP5 fragment (the immunogen) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 61 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CRMP5 antibody (ab77158) at 5 µg/ml

    Lane 1 : CRMP5 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 61 kDa
    Observed band size : 61 kDa

Anti-CRMP5 antibody (ab77158)参考文献

ab77158 has not yet been referenced specifically in any publications.

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