Anti-CRMP3抗体(ab36217)
Key features and details
- Rabbit polyclonal to CRMP3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CRMP3抗体
参阅全部 CRMP3 一抗 -
描述
兔多克隆抗体to CRMP3 -
宿主
Rabbit -
特异性
No cross reactivity was detected with CRMP1, CRMP2, CRMP4 or CRMP5. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
A 15 residue synthetic peptide derived from C-terminal domain of human CRMP3.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab36217于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000. Predicted molecular weight: 65 kDa.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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WB
1/2000. Predicted molecular weight: 65 kDa. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. -
序列相似性
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 10570 Human
- Omim: 608407 Human
- SwissProt: O14531 Human
- Unigene: 100058 Human
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别名
- Collapsin response mediator protein 3 antibody
- CRMP 3 antibody
- CRMP-3 antibody
see all
图片
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All lanes : Anti-CRMP3 antibody (ab36217)
Lane 1 : E.coli transformed with cDNA of human CRMP1
Lane 2 : E.coli transformed with cDNA of human CRMP2
Lane 3 : E.coli transformed with cDNA of human CRMP3
Lane 4 : E.coli transformed with cDNA of human CRMP4
Lane 5 : E.coli transformed with cDNA of human CRMP5
Predicted band size: 65 kDa
Observed band size: 65 kDa -
IHC image of ab36217 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36217, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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Datasheet download
文献 (2)
ab36217 被引用在 2 文献中.
- Lin YS et al. Collapsin response mediator protein 5 (CRMP5) causes social deficits and accelerates memory loss in an animal model of Alzheimer's disease. Neuropharmacology 157:107673 (2019). PubMed: 31233825
- Hou ST et al. Collapsin response mediator protein 3 deacetylates histone H4 to mediate nuclear condensation and neuronal death. Sci Rep 3:1350 (2013). ChIP ; Mouse . PubMed: 23443259