Anti-CRMP2 (phospho T514)抗体(ab85934)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-CRMP2 (phospho T514)抗体
参阅全部 CRMP2 一抗 -
描述兔多克隆抗体to CRMP2 (phospho T514)
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宿主Rabbit
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经测试应用适用于: ICC/IF, WB, IPmore details
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种属反应性与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human CRMP2, phosphorylated at T514.
(Peptide available as ab99478.)
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阳性对照
- This antibody gave a positive signal in human, mouse and rat brain tissue lysates.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
应用
Our Abpromise guarantee covers the use of ab85934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). | |
| IP | Use a concentration of 5 µg/ml. |
靶标
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功能Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
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组织特异性Ubiquitous.
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序列相似性Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
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翻译后修饰3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
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细胞定位Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 1808 Human
- Entrez Gene: 12934 Mouse
- Entrez Gene: 25416 Rat
- Omim: 602463 Human
- SwissProt: Q16555 Human
- SwissProt: O08553 Mouse
- SwissProt: P47942 Rat
- Unigene: 173381 Human
see all -
别名
- Collapsin response mediator protein 2 antibody
- Collapsin response mediator protein antibody
- Collapsin response mediator protein hCRMP 2 antibody
see all
图片
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Western blot - CRMP2 (phospho T514) antibody (ab85934)All lanes : Anti-CRMP2 (phospho T514) antibody (ab85934) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
Lane 5 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
Lane 6 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
Lane 7 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
Lane 8 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
Lane 9 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
Lysates/proteins at 10 µg per lane.
Blocking peptides at 1 µg/ml per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62,70 kDa (why is the actual band size different from the predicted?)
Exposure time: 30 seconds
The predicted molecular weight of is 62-kDa (SwissProt), however in mouse and rat brain tissue lysates, we observe a band at 70-kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - CRMP2 (phospho T514) antibody (ab85934)ICC/IF image of ab85934 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85934 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunoprecipitation - Anti-CRMP2 (phospho T514) antibody (ab85934)CRMP2 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to CRMP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab85934.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 70kDa; CRMP2
实验方案
文献
This product has been referenced in:
- Akama K et al. Proteomic identification of differentially expressed genes in neural stem cells and neurons differentiated from embryonic stem cells of cynomolgus monkey (Macaca fascicularis) in vitro. Biochim Biophys Acta 1814:265-76 (2011). WB ; Monkey . Read more (PubMed: 21047566) »
客户评价及客户问答
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