概述

  • 产品名称Anti-CRMP2 (phospho T514)抗体
    参阅全部 CRMP2 一抗
  • 描述
    兔多克隆抗体to CRMP2 (phospho T514)
  • 特异性ab62478 detects endogenous levels of CRMP2 only when phosphorylated at threonine 514.
  • 经测试应用适用于: ELISA, ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human CRMP2 around the phosphorylation site of threonine 514 (T-V-TP-P-A).

  • 阳性对照
    • Human brain tissue and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab62478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

靶标

  • 功能Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • 翻译后修饰3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Collapsin response mediator protein 2 antibody
    • Collapsin response mediator protein antibody
    • Collapsin response mediator protein hCRMP 2 antibody
    • CRAM antibody
    • CRMP 2 antibody
    • CRMP-2 antibody
    • CRMP2 antibody
    • DHPRP 2 antibody
    • DHPRP2 antibody
    • Dihydropyrimidinase 2 antibody
    • Dihydropyrimidinase like 2 antibody
    • Dihydropyrimidinase like 2 long form antibody
    • Dihydropyrimidinase related protein 2 antibody
    • Dihydropyrimidinase-related protein 2 antibody
    • DPYL 2 antibody
    • DPYL2 antibody
    • DPYL2_HUMAN antibody
    • DPYSL 2 antibody
    • Dpysl2 antibody
    • DRP-2 antibody
    • DRP2 antibody
    • Musunc 33 antibody
    • Musunc33 antibody
    • N2A3 antibody
    • TOAD 64 antibody
    • TOAD64 antibody
    • ULIP 2 protein antibody
    • ULIP-2 antibody
    • Ulip2 antibody
    • Unc-33-like phosphoprotein 2 antibody
    see all

Anti-CRMP2 (phospho T514) antibody 图像

  • ab62478, at 1/50 dilution, staining CRMP2 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.
  • ab62478, at 1/500 dilution, staining CRMP2 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • Anti-CRMP2 (phospho T514) antibody (ab62478) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of RPE cells, staining CRMP2 (phospho T514) with ab62478. Cells were fixed in cold methanol and incubated with primary antibody, followed by an AlexaFluor®488-conjugated anti rabbit IgG.

Anti-CRMP2 (phospho T514) antibody (ab62478)参考文献

This product has been referenced in:
  • Lim NK  et al. Localized changes to glycogen synthase kinase-3 and collapsin response mediator protein-2 in the Huntington's disease affected brain. Hum Mol Genet N/A:N/A (2014). WB ; Mouse . Read more (PubMed: 24634145) »
  • Sutinen EM  et al. Interleukin-18 alters protein expressions of neurodegenerative diseases-linked proteins in human SH-SY5Y neuron-like cells. Front Cell Neurosci 8:214 (2014). WB ; Human . Read more (PubMed: 25147500) »

See all 6 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab62478.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"