概述

  • 产品名称Anti-CRMP2抗体
    参阅全部 CRMP2 一抗
  • 描述
    兔多克隆抗体to CRMP2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Xenopus laevis, Zebrafish, Orangutan
  • 免疫原

    Synthetic peptide:

    DFVYKRIKARSRLAELRG

    conjugated to KLH, corresponding to amino acids 476-493 of Human CRMP2

  • 阳性对照
    • Hela whole cell lysate; mouse brain extract

性能

应用

Our Abpromise guarantee covers the use of ab62661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

靶标

  • 功能Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • 翻译后修饰3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Collapsin response mediator protein 2 antibody
    • Collapsin response mediator protein antibody
    • Collapsin response mediator protein hCRMP 2 antibody
    • CRAM antibody
    • CRMP 2 antibody
    • CRMP-2 antibody
    • CRMP2 antibody
    • DHPRP 2 antibody
    • DHPRP2 antibody
    • Dihydropyrimidinase 2 antibody
    • Dihydropyrimidinase like 2 antibody
    • Dihydropyrimidinase like 2 long form antibody
    • Dihydropyrimidinase related protein 2 antibody
    • Dihydropyrimidinase-related protein 2 antibody
    • DPYL 2 antibody
    • DPYL2 antibody
    • DPYL2_HUMAN antibody
    • DPYSL 2 antibody
    • Dpysl2 antibody
    • DRP-2 antibody
    • DRP2 antibody
    • Musunc 33 antibody
    • Musunc33 antibody
    • N2A3 antibody
    • TOAD 64 antibody
    • TOAD64 antibody
    • ULIP 2 protein antibody
    • ULIP-2 antibody
    • Ulip2 antibody
    • Unc-33-like phosphoprotein 2 antibody
    see all

Anti-CRMP2 antibody 图像

  • All lanes : Anti-CRMP2 antibody (ab62661) at 1/10000 dilution

    Lane 1 : Mouse brain (S1 fraction)
    Lane 2 : Rat brain (S1 fraction)
    Lane 3 : HeLa cells
    Lane 4 : HEK-293T cells transfected with human CRMP2
    Lane 5 : Untransfected HEK-293T cells
    Lane 6 : Mouse brain (S1 fraction): with CRMP2 immunizing peptide at 10 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate
    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • All lanes : Anti-CRMP2 antibody (ab62661) at 1/10000 dilution

    Lane 1 : Cell lysate prepared from rat cortex.
    Lane 2 : to 10 : Rat hippocampal homogenates.

    Lysates/proteins at 5 µg per lane.

    Secondary
    Alexafluor 680 conjugated mouse polyclonal to rabbit at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 63 kDa


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Dr Martin Broadstock

    See Abreview

Anti-CRMP2 antibody (ab62661)参考文献

This product has been referenced in:
  • Hori M  et al. PACAP38 differentially effects genes and CRMP2 protein expression in ischemic core and penumbra regions of permanent middle cerebral artery occlusion model mice brain. Int J Mol Sci 15:17014-34 (2014). WB ; Mouse . Read more (PubMed: 25257527) »
  • Braunschweig D  et al. Autism-specific maternal autoantibodies recognize critical proteins in developing brain. Transl Psychiatry 3:e277 (2013). Read more (PubMed: 23838888) »

See all 4 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Hippocampal lysates - mouse)
Loading amount 5 µg
Specification Hippocampal lysates - mouse
Gel Running Conditions Reduced Denaturing (10% gel, 5 micrograms protein loaded)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Martin Broadstock

Verified customer

提交于 May 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"