Anti-CRMP1抗体(ab76995)

概述

  • 产品名称Anti-CRMP1抗体
    参阅全部 CRMP1 一抗
  • 描述
    小鼠单克隆抗体to CRMP1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment: HVVPEPGSSL LTSFEKWHEA ADTKSCCDYS LHVDITSWYD GVREELEVLV QDKGVNSFQV YMAYKDVYQM SDSQLYEAFT FLKGLGAVIL, corresponding to amino acids 221-310 of human CRMP1 (NP_001014809) with a 26 kDa tag

  • 阳性对照
    • IMR-32 cell lysate, immunogen

性能

应用

Our Abpromise guarantee covers the use of ab76995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 62 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.
  • 组织特异性Brain.
  • 序列相似性Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes and the mitotic spindle during metaphase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Collapsin response mediator protein 1 antibody
    • CRMP 1 antibody
    • CRMP-1 antibody
    • Crmp1 antibody
    • Dihydropyrimidinase like 1 antibody
    • Dihydropyrimidinase related protein 1 antibody
    • Dihydropyrimidinase-related protein 1 antibody
    • DPYL1_HUMAN antibody
    • DPYSL1 antibody
    • DRP 1 antibody
    • DRP-1 antibody
    • DRP1 antibody
    • ULIP-3 antibody
    • Ulip3 antibody
    • Unc-33-like phosphoprotein 3 antibody
    see all

Anti-CRMP1 antibody 图像

  • Anti-CRMP1 antibody (ab76995) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 62 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-CRMP1 antibody (ab76995) at 5 µg/ml + IMR-32 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 62 kDa
    Observed band size : 62 kDa

Anti-CRMP1 antibody (ab76995)参考文献

ab76995 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Hippocampus)
Loading amount 5 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"