概述

  • 产品名称
  • 描述
    兔多克隆抗体to CRM1
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 1025-1071 (FAGEDTSDLF LEEREIALRQ ADEEKHKRQM SVPGIFNPHE IPEEMCD) of human CRM1 (NP_003391.1).

  • 阳性对照
    • Human breast tumor; mouse squamous cell carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab84375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明
    IHC-P: 1/100 - 1/500.
    Epitope exposure is recommended.
    Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.
    • 组织特异性
      Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.
    • 序列相似性
      Belongs to the exportin family.
      Contains 10 HEAT repeats.
      Contains 1 importin N-terminal domain.
    • 翻译后修饰
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位
      Cytoplasm. Nucleus > nucleoplasm. Nucleus > Cajal body. Nucleus > nucleolus. Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Chromosome region maintenance 1 protein homolog antibody
      • CRM 1 antibody
      • CRM1 homolog antibody
      • DKFZp686B1823 antibody
      • emb antibody
      • Exp 1 antibody
      • Exp1 antibody
      • Exportin 1 antibody
      • Exportin-1 antibody
      • Exportin1 antibody
      • XPO 1 antibody
      • xpo1 antibody
      • XPO1_HUMAN antibody
      see all

    图片

    • ab84375, at a 1/250 dilution, staining CRM1 in formalin fixed, paraffin embedded human breast tumor section by immunohistochemistry.
      Detection: DAB staining
    • ab84375, at a 1/250 dilution, staining CRM1 in formalin fixed, paraffin embedded mouse squamous cell carcinoma by immunohistochemistry. Detection: DAB staining

    文献

    This product has been referenced in:
    • Heerma van Voss MR  et al. Nuclear DDX3 expression predicts poor outcome in colorectal and breast cancer. Onco Targets Ther 10:3501-3513 (2017). Read more (PubMed: 28761359) »

    See 1 Publication for this product

    客户评价及客户问答

    Thank you for your enquiry. I wish to sincerely apologize for the delay.

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    Thank you for your enquiry.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any CRM1 antibody tested in the s...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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