• 产品名称
  • 描述
    兔多克隆抗体to CRLR
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human CRLR.

    (Peptide available as ab99160.)

  • 阳性对照
    • This antibody gave a positive signal in human liver tissue and A549 whole cell lysates. IHC-P: human lung tissue sections.



Our Abpromise guarantee covers the use of ab84467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 53 kDa).


  • 相关性
    CRLR is a receptor for calcitonin gene related peptide type 1. The activity of CRLR is mediated by G proteins which activate adenylyl cyclase. CRLR expression has been reported in brain, lung, blood vessel, liver, and intestinal tract. ESTs have been isolated from B Cell/lung/testis, bone marrow, embryo, lung, and synovium libraries.
  • 细胞定位
    Cell Membrane; Multi-pass membrane protein.
  • 数据库链接
  • 别名
    • Calcitonin gene related peptide type 1 receptor antibody
    • Calcitonin receptor like antibody
    • Calcitonin receptor like receptor antibody
    • CALCRL antibody
    • CGRP type 1 receptor antibody
    • CGRPR antibody
    • CRLR antibody
    see all

Anti-CRLR antibody 图像

  • All lanes : Anti-CRLR antibody (ab84467) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)

    Exposure time : 8 minutes
  • IHC image of CRLR staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84467, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CRLR antibody (ab84467)参考文献

ab84467 has not yet been referenced specifically in any publications.

Product Wall

Thanks for your call today and for letting us know about the trouble with ab84467. As we discussed, I would recommend using a higher concentration of secondary antibody, and checking that the other components of the assay have been working with...

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