概述

  • 产品名称
  • 描述
    兔多克隆抗体to CRLR
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (DGNWFRHPAS NRTWTNYTQC NVNTHEKVKT ALNLFYLTII GHGLSIASLL) of human CRLR (NP_005786).

  • 阳性对照
    • Fetal muscle lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-CRLR antibody (ab83697) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa

实验方案

文献

ab83697 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Mouse Tissue sections (Skin)
Specification
Skin
Permeabilization
Yes - 0,3% TritonX
Fixative
Perfusion fixed with paraformaldehyde and picric acid
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提交于 Aug 11 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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