概述

  • 产品名称
  • 描述
    兔多克隆抗体to CrkRS
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    KLH-conjugated synthetic peptide selected from the sequence of Human CrkRS within amino acids 1-100.

  • 阳性对照
    • Mouse liver tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab37914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 145 kDa (predicted molecular weight: 164 kDa).

靶标

  • 功能
    Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
  • 组织特异性
    Ubiquitously expressed.
  • 疾病相关
    Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.
  • 序列相似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylation at Thr-893 increases kinase activity.
  • 细胞定位
    Nucleus. Nucleus speckle. Colocalized with nuclear speckles throughout interphase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • arginine/serine-rich antibody
    • Cdc2 related kinase arginine/serine rich antibody
    • CDC2 related protein kinase 7 antibody
    • Cdc2-related kinase antibody
    • CDC2-related protein kinase 7 antibody
    • Cdk12 antibody
    • CDK12_HUMAN antibody
    • Cell division cycle 2 related protein kinase 7 antibody
    • Cell division cycle 2-related protein kinase 7 antibody
    • Cell division protein kinase 12 antibody
    • CRK7 antibody
    • CRKR antibody
    • CrkRS antibody
    • Cyclin-dependent kinase 12 antibody
    • EC 2.7.11.22 antibody
    • hCDK12 antibody
    • kiaa0904 antibody
    • Pksc antibody
    see all

图片

实验方案

文献

This product has been referenced in:
  • Natrajan R  et al. Characterization of the genomic features and expressed fusion genes in micropapillary carcinomas of the breast. J Pathol 232:553-65 (2014). Read more (PubMed: 24395524) »

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Jun 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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