RabMAb

Anti-CrkL (phospho Y207)抗体[EP270Y] (ab52908)

概述

  • 产品名称Anti-CrkL (phospho Y207)抗体[EP270Y]
    参阅全部 CrkL 一抗
  • 描述
    兔单克隆抗体[EP270Y] to CrkL (phospho Y207)
  • 特异性ab52908 only detects CrkL phosphorylated on Tyrosine 207.
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic phospho peptide corresponding to residues surrounding Tyr207 of human CrkL

  • 阳性对照
    • WB: K562 cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab52908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 34 kDa).
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt or IHC-P.
  • 靶标

    Anti-CrkL (phospho Y207) antibody [EP270Y] 图像

    • All lanes : Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908) at 1/50000 dilution

      Lane 1 : (A) K562 cell lysates untreated
      Lane 2 : (B) K562 cell lysates treated with AP

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908)参考文献

    This product has been referenced in:
    • Li J  et al. Inhibition of 32Dp210 cells harboring T315I mutation by a novel derivative of emodin correlates with down-regulation of BCR-ABL and its downstream signaling pathways. J Cancer Res Clin Oncol N/A:N/A (2014). Human . Read more (PubMed: 25217883) »
    • Yang TY  et al. A multiple reaction monitoring (MRM) method to detect Bcr-Abl kinase activity in CML using a peptide biosensor. PLoS One 8:e56627 (2013). WB ; Human . Read more (PubMed: 23437189) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"