概述

  • 产品名称Anti-CrkL抗体[Y243]
    参阅全部 CrkL 一抗
  • 描述
    兔单克隆抗体[Y243] to CrkL
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the SH2 domain, near the N-term of human CrkL

  • 阳性对照
    • K562 cell lysate and human breast carcinoma
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab32126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/50 - 1/100.
ICC 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能May mediate the transduction of intracellular signals.
    • 序列相似性Belongs to the CRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Crk L antibody
      • Crk like protein antibody
      • Crk-like protein antibody
      • Crkl antibody
      • CRKL_HUMAN antibody
      • Crkol antibody
      • HGNC:2363 antibody
      • Oncogene CrkL antibody
      • V crk avian sarcoma virus CT10 oncogene homolog like antibody
      • v crk sarcoma virus CT10 oncogene homolog (avian) like antibody
      • V crk sarcoma virus CT10 oncogene homolog avian like antibody
      see all

    Anti-CrkL antibody [Y243] 图像

    • Anti-CrkL antibody [Y243] (ab32126) at 1/1000 dilution + K562 cell lysate

      Predicted band size : 33 kDa
      Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    • ab32126, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue

    Anti-CrkL antibody [Y243] (ab32126)参考文献

    This product has been referenced in:
    • Wang J  et al. SCG10 promotes non-amyloidogenic processing of amyloid precursor protein by facilitating its trafficking to the cell surface. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23863461) »
    • Natsume H  et al. The CRKL gene encoding an adaptor protein is amplified, overexpressed, and a possible therapeutic target in gastric cancer. J Transl Med 10:97 (2012). WB, IHC-P ; Human . Read more (PubMed: 22591714) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your inquiry. I have confirmed that neither of these two anti-CrkL antibodies, ab32018 or ab32126, bind within the region a.a. 123 - 183 (the first SH3 domain). I apologize but it doesn't look like any of the anti-CrkL ant...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"