Synthetic non-phosphopeptide derived from human CrkL around the phosphorylation site of tyrosine 207 (H-A-YP-A-Q).
- Human lung carcinoma tissue, extracts from 293 cells, HuvEc cells.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/300 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
||1/50 - 1/100.
||1/500 - 1/1000.
Anti-CrkL antibody 图像
Immunocytochemistry/ Immunofluorescence - CrkL antibody (ab63491)
This image shows HuvEc cells stained with ab63491 at 1/500 dilution; with and without the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CrkL antibody (ab63491)
This image shows human lung carcinoma tissue stained with ab63491 at 1/50 dilution; with and without the immunising peptide.
Western blot - CrkL antibody (ab63491)
All lanes : Anti-CrkL antibody (ab63491) at 1/300 dilution
Lane 1 : Extracts from 293 cells (5-30µg total protein)
Lane 2 : Extracts from 293 cells (5-30µg total protein) and the immunising peptide (5-10µg).
Predicted band size : 34 kDa
Observed band size : 34 kDa
Anti-CrkL antibody (ab63491)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"