RabMAb

Anti-Crk p38 (phospho Y221)抗体[EPR269Y] (ab76227)

概述

  • 产品名称Anti-Crk p38 (phospho Y221)抗体[EPR269Y]
    参阅全部 Crk p38 一抗
  • 描述
    兔单克隆抗体[EPR269Y] to Crk p38 (phospho Y221)
  • 特异性This antibody detects proteins phosphorylated at tyrosine 221.
  • 经测试应用适用于: WB, ICCmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to regions surrounding Tyrosine 221 of human Crk p38

  • 阳性对照
    • K562 cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR269Y
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab76227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 34 kDa.
ICC 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
    • 序列相似性Belongs to the CRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • 结构域The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
      The SH2 domain mediates interaction with SHB.
    • 翻译后修饰Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
      Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
    • 细胞定位Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Adapter molecule crk antibody
      • Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
      • CRK antibody
      • CRK isoform 2 antibody
      • CRK isoform II antibody
      • CRK_HUMAN antibody
      • CRKII antibody
      • FLJ38130 antibody
      • OTTHUMP00000115366 antibody
      • OTTHUMP00000198330 antibody
      • p38 antibody
      • Proto oncogene C crk antibody
      • Proto-oncogene C-crk antibody
      • v crk avian sarcoma virus CT10 oncogene homolog antibody
      • v crk sarcoma virus CT10 oncogene homolog antibody
      • v crk sarcoma virus CT10 oncogene homolog (avian) antibody
      see all

    Anti-Crk p38 (phospho Y221) antibody [EPR269Y] 图像

    • All lanes : Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (ab76227) at 1/2000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : K562 cell lysate treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 34 kDa

    Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (ab76227)参考文献

    This product has been referenced in:
    • Nieto-Pelegrin E  et al. Crk adaptors negatively regulate actin polymerization in pedestals formed by enteropathogenic Escherichia coli (EPEC) by binding to Tir effector. PLoS Pathog 10:e1004022 (2014). WB ; Human . Read more (PubMed: 24675776) »
    • Li XN  et al. The p38 MAPK inhibitor JLU1124 inhibits the inflammatory response induced by lipopolysaccharide through the MAPK-NF-?B pathway in RAW264.7 macrophages. Int Immunopharmacol 17:785-92 (2013). Read more (PubMed: 24070708) »

    See all 3 Publications for this product

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