Anti-Crk p38 (phospho Y221)抗体(ab51222)

概述

  • 产品名称Anti-Crk p38 (phospho Y221)抗体
    参阅全部 Crk p38 一抗
  • 描述
    兔多克隆抗体to Crk p38 (phospho Y221)
  • 特异性This antibody detects endogenous levels of CRK isoform II only when phosphorylated at tyrosine 221.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A synthesized phosphopeptide derived from human CRK isoform II around the phosphorylation site of tyrosine 221 (G-P-YP-A-Q).

  • 阳性对照
    • Extracts of COS7 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/300 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).
ELISA 1/40000.

靶标

  • 功能The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
  • 序列相似性Belongs to the CRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • 结构域The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
    The SH2 domain mediates interaction with SHB.
  • 翻译后修饰Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
    Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
  • 细胞定位Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adapter molecule crk antibody
    • Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
    • CRK antibody
    • CRK isoform 2 antibody
    • CRK isoform II antibody
    • CRK_HUMAN antibody
    • CRKII antibody
    • FLJ38130 antibody
    • OTTHUMP00000115366 antibody
    • OTTHUMP00000198330 antibody
    • p38 antibody
    • Proto oncogene C crk antibody
    • Proto-oncogene C-crk antibody
    • v crk avian sarcoma virus CT10 oncogene homolog antibody
    • v crk sarcoma virus CT10 oncogene homolog antibody
    • v crk sarcoma virus CT10 oncogene homolog (avian) antibody
    see all

Anti-Crk p38 (phospho Y221) antibody 图像

  • All lanes : Anti-Crk p38 (phospho Y221) antibody (ab51222) at 1/300 dilution

    Lane 1 : Extracts of cos7 cells treated with phosphopeptide
    Lane 2 : Extracts of cos7 cells


    Predicted band size : 34 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)

Anti-Crk p38 (phospho Y221) antibody (ab51222)参考文献

ab51222 has not yet been referenced specifically in any publications.

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