Anti-CRISP3抗体[LV-2A2] (ab62225)

概述

  • 产品名称Anti-CRISP3抗体[LV-2A2]
    参阅全部 CRISP3 一抗
  • 描述
    小鼠单克隆抗体[LV-2A2] to CRISP3
  • 经测试应用适用于: Flow Cyt, Competitive ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Genetic immunisation with cDNA encoding human CRISP3

  • 阳性对照
    • BOSC23 cells transfected with an expression vector encoding human CRISP3.
  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. Genetic immunization was with cDNA encoding human CRISP3 and selection was based on recognition of the complete native protein expressed on transfected mammalian cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. Genetic immunization was with cDNA encoding human CRISP3 and selection was based on recognition of the complete native protein expressed on transfected mammalian cells.
  • 克隆单克隆
  • 克隆编号LV-2A2
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1.2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Competitive ELISA 1/200 - 1/400.

靶标

  • 组织特异性Salivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.
  • 序列相似性Belongs to the CRISP family.
  • 细胞定位Secreted. In neutrophils, localized in specific granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Aeg 2 antibody
    • Aeg2 antibody
    • CRIS3_HUMAN antibody
    • CRISP 3 antibody
    • CRISP-3 antibody
    • Crisp3 antibody
    • CRS 3 antibody
    • CRS3 antibody
    • Cysteine rich secretory protein 3 antibody
    • Cysteine-rich secretory protein 3 antibody
    • dJ442L6.3 antibody
    • MGC126588 antibody
    • OTTHUMP00000016590 antibody
    • OTTHUMP00000039911 antibody
    • SGP 28 antibody
    • SGP28 antibody
    • SGP28 protein antibody
    • Specific granule protein (28 kDa) antibody
    • Specific granule protein of 28 kDa antibody
    see all

Anti-CRISP3 antibody [LV-2A2] 图像

  • FACS analysis of BOSC23 cells using CRISP3 antibody [LV-2A2] (ab62225). BOSC23 cells were transiently transfected with an expression vector encoding either CRISP3 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of ab62225 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CRISP3 transfected cells.

Anti-CRISP3 antibody [LV-2A2] (ab62225)参考文献

ab62225 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab62225.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"