重组
RabMAb

Anti-Cripto1抗体[EPNCIR106A] (ab108391)

概述

性能

应用

Our Abpromise guarantee covers the use of ab108391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP 1/10 - 1/100.
ICC 1/500.

For unpurified use at 1/100 -1/250. 

Flow Cyt Use at an assay dependent concentration.

靶标

  • 功能
    Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
  • 组织特异性
    Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
  • 序列相似性
    Contains 1 EGF-like domain.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CR antibody
    • CRGF antibody
    • Cripto 1 antibody
    • Cripto 1 growth factor antibody
    • cripto antibody
    • Cripto-1 growth factor antibody
    • Cripto1 growth factor antibody
    • Epidermal growth factor like cripto protein CR1 antibody
    • Epidermal growth factor-like cripto protein CR1 antibody
    • TDGF 1 antibody
    • TDGF1 antibody
    • TDGF1_HUMAN antibody
    • Teratocarcinoma derived growth factor 1 antibody
    • Teratocarcinoma-derived growth factor 1 antibody
    see all

Anti-Cripto1 antibody [EPNCIR106A] 图像

  • Anti-Cripto1 antibody [EPNCIR106A] (ab108391) at 1/2000 dilution (purified) + NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 21 kDa

    Blocking and diluting buffer: 5% NFDM/TBST

  • Flow Cytometry analysis of NCCIT(human pluripotent embryonal carcinoma) cells labeling Cripto1 with purified ab108391 
    at 1/2200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • ab108391 (purified) at 1:30 dilution (2ug) immunoprecipitating Cripto1 in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate.
    Lane 1 (input): NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab108391 & NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108391 in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.

  • Immunocytochemistry/ Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Cripto1 with Purified ab108391 at 1:500 dilution (1.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Anti-Cripto1 antibody [EPNCIR106A] (ab108391) at 1/1000 dilution (unpurified) + NCCIT cell lysate at 10 µg

    Predicted band size : 21 kDa
    Observed band size : 21 kDa

Anti-Cripto1 antibody [EPNCIR106A] (ab108391)参考文献

ab108391 has not yet been referenced specifically in any publications.

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