重组
RabMAb

Anti-Cripto1抗体[EPNCIR106A] (ab108391)

概述

  • 产品名称
    Anti-Cripto1抗体[EPNCIR106A]
    参阅全部 Cripto1 一抗
  • 描述
    兔单克隆抗体[EPNCIR106A] to Cripto1
  • 经测试应用
    适用于: WB, IP, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human Cripto1. The exact sequence is proprietary.
    Database link: P13385
    (Peptide available as ab193609)

  • 阳性对照
    • WB and IP: NCCIT cell lysate. Flow Cytometry and ICC/IF: NCCIT cells.
  • 常规说明

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP 1/10 - 1/100.
ICC 1/500.

For unpurified use at 1/100 -1/250. 

Flow Cyt Use at an assay dependent concentration.

靶标

  • 功能
    Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
  • 组织特异性
    Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
  • 序列相似性
    Contains 1 EGF-like domain.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CR antibody
    • CRGF antibody
    • Cripto 1 antibody
    • Cripto 1 growth factor antibody
    • cripto antibody
    • Cripto-1 growth factor antibody
    • Cripto1 growth factor antibody
    • Epidermal growth factor like cripto protein CR1 antibody
    • Epidermal growth factor-like cripto protein CR1 antibody
    • TDGF 1 antibody
    • TDGF1 antibody
    • TDGF1_HUMAN antibody
    • Teratocarcinoma derived growth factor 1 antibody
    • Teratocarcinoma-derived growth factor 1 antibody
    see all

图片

  • Anti-Cripto1 antibody [EPNCIR106A] (ab108391) at 1/2000 dilution (purified) + NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 21 kDa

    Blocking and diluting buffer: 5% NFDM/TBST

  • Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling Cripto1 with purified ab108391 
    at 1/2200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • ab108391 (purified) at 1:30 dilution (2ug) immunoprecipitating Cripto1 in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate.
    Lane 1 (input): NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab108391 & NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108391 in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.

  • Immunocytochemistry/ Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Cripto1 with Purified ab108391 at 1:500 dilution (1.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Anti-Cripto1 antibody [EPNCIR106A] (ab108391) at 1/1000 dilution (unpurified) + NCCIT cell lysate at 10 µg

    Predicted band size : 21 kDa
    Observed band size : 21 kDa

文献

ab108391 has not yet been referenced specifically in any publications.

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