概述

  • 产品名称Anti-Cripto1抗体
    参阅全部 Cripto1 一抗
  • 描述
    兔多克隆抗体to Cripto1
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Cripto.

    (Peptide available as ab23310.)

  • 阳性对照
    • Human Embryonic Stem Cells, Cripto1 Recombinant Protein

性能

应用

Our Abpromise guarantee covers the use of ab19917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 21 kDa).

靶标

  • 功能Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
  • 组织特异性Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
  • 序列相似性Contains 1 EGF-like domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CR antibody
    • CRGF antibody
    • Cripto 1 antibody
    • Cripto 1 growth factor antibody
    • cripto antibody
    • Cripto-1 growth factor antibody
    • Cripto1 growth factor antibody
    • Epidermal growth factor like cripto protein CR1 antibody
    • Epidermal growth factor-like cripto protein CR1 antibody
    • TDGF 1 antibody
    • TDGF1 antibody
    • TDGF1_HUMAN antibody
    • Teratocarcinoma derived growth factor 1 antibody
    • Teratocarcinoma-derived growth factor 1 antibody
    see all

Anti-Cripto1 antibody 图像

  • ICC/IF image of ab19917 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19917, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-Cripto1 antibody (ab19917) at 1 µg/ml

    Lane 1 : Human Embryonic Stem Cell Lysate at 20 µg
    Lane 2 : Cripto protein at 0.1 µg
    Lane 3 : Human Embryonic Stem Cell Lysate at 20 µg with Human Cripto1 peptide (ab23310) at 1 µg/ml
    Lane 4 : Cripto protein at 0.1 µg with Human Cripto1 peptide (ab23310) at 1 µg/ml

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Predicted band size : 21 kDa

    The 17 kDa band detected in human embryonic stem cells may correspond with Cripto that has had its signal sequence cleaved. The Cripto bands were blocked with the immunizing peptide, ab23310.

  • Anti-Cripto antibody, ab19917, stained the membrane of human embryonic stem cells at a dilution of 1/100.
  • The image shows staining of human pancreas tissue. Staining was nuclear in some islet cells and cytoplasmic in some cells of the acini. Antigen retrieval was performed using Citrate at pH6. Nuclear localization of Cripto protein has been described in the literature: Gong YP et al. Eur J Surg Oncol, 2006.

Anti-Cripto1 antibody (ab19917)参考文献

This product has been referenced in:
  • Spike BT  et al. CRIPTO/GRP78 Signaling Maintains Fetal and Adult Mammary Stem Cells Ex Vivo. Stem Cell Reports 2:427-39 (2014). ICC/IF ; Human . Read more (PubMed: 24749068) »
  • Spiller CM  et al. Endogenous Nodal signaling regulates germ cell potency during mammalian testis development. Development 139:4123-32 (2012). IHC-P ; Mouse . Read more (PubMed: 23034635) »

See all 5 Publications for this product

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I am sorry we do not sell the human embryonic stem cells.

As far as we are aware, this antibody has not been tested in other sample types. I can recommend the customer try a literature search to obtain ...

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