Anti-CRF1/CRHR1抗体(ab77686)
Key features and details
- Goat polyclonal to CRF1/CRHR1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-CRF1/CRHR1抗体
参阅全部 CRF1/CRHR1 一抗 -
描述
山羊多克隆抗体to CRF1/CRHR1 -
宿主
Goat -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human CRF1/CRHR1 aa 107-117 (internal sequence).
Sequence:C-NEEKKSKVHYH
Database link: NP_004373.2 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab77686 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77686于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).
1 hour primary incubation is recommended for this product. |
|
ICC/IF |
Use a concentration of 0.3 µg/ml.
|
说明 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa). 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 0.3 µg/ml. |
靶标
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功能
This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
组织特异性
Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. -
翻译后修饰
C-terminal Ser or Thr residues may be phosphorylated. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1394 Human
- Entrez Gene: 12921 Mouse
- Entrez Gene: 58959 Rat
- Omim: 122561 Human
- SwissProt: P34998 Human
- SwissProt: P35347 Mouse
- SwissProt: P35353 Rat
- Unigene: 417628 Human
see all -
别名
- corticotropin releasing factor receptor antibody
- Corticotropin releasing factor type 1 receptor antibody
- corticotropin releasing hormone receptor 1 antibody
see all
图片
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All lanes : Anti-CRF1/CRHR1 antibody (ab77686) at 1 µg/ml
Lane 1 : Human colon tissue lysate in RIPA buffer
Lane 2 : Human cerebellum tissue lysate in RIPA buffer
Lane 3 : Rat brain tissue lysate in RIPA buffer
Lane 4 : Mouse spinal cord tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 48 kDaIncubated with the primary antibody for 1 hour. Detected by chemiluminescence.
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Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling CRF1 with ab77686 at 10µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.15% Triton X-100. Anti-goat Alexa Fluor® 488 antibody was used as the secondary antibody (green) showing cytoplasmic, and vesicle staining. Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG (10μg/mL) followed by Alexa Fluor® 488 secondary antibody (2μg/mL).
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Immunocytochemistry/immunofluorescence analysis of Neuro2a cells labelling CRF1 with ab77686 at 10µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.15% Triton X-100. Anti-goat Alexa Fluor® 488 antibody at 2ug/ml was used as the secondary antibody (green) showing plasma membrane, and vesicle staining. Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor® 488 secondary antibody (2ug/ml).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab77686 被引用在 5 文献中.
- Yamanaka-Takaichi M et al. Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa. Int J Mol Sci 22:N/A (2021). PubMed: 33803422
- You X et al. Urocortins exhibit differential effects on PGE2 and PGF2α output via CRHR2 in human myometrium. Reproduction 162:11-20 (2021). PubMed: 33929340
- Ayyadurai S et al. Frontline Science: Corticotropin-releasing factor receptor subtype 1 is a critical modulator of mast cell degranulation and stress-induced pathophysiology. J Leukoc Biol 102:1299-1312 (2017). PubMed: 28684600
- Wierzbicka JM et al. Differentiation of Keratinocytes Modulates Skin HPA Analog. J Cell Physiol 232:154-66 (2017). PubMed: 27061711
- Wierzbicka JM et al. Bioactive forms of vitamin D selectively stimulate the skin analog of the hypothalamus-pituitary-adrenal axis in human epidermal keratinocytes. Mol Cell Endocrinol 437:312-322 (2016). PubMed: 27524410