概述

  • 产品名称Anti-CREM抗体
    参阅全部 CREM 一抗
  • 描述
    小鼠单克隆抗体to CREM
  • 经测试应用适用于: Flow Cyt, WBmore details
  • 种属反应性
    与反应: Human, Recombinant Fragment
  • 免疫原

    Recombinant fragment: ATGDMPTYQI RAPTAALPQG VVMAASPGSL HSPQQLAEEA TRKRELRLMK NREAAKECRR RKKEYVKCLE SRVAVLEVQN KKLIEELETL KDICSPKTDY , corresponding to amino acids 201-301 of Human CREM

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab54625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

靶标

  • 功能Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.
  • 组织特异性Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.
  • 序列相似性Belongs to the bZIP family.
    Contains 1 bZIP (basic-leucine zipper) domain.
    Contains 1 KID (kinase-inducible) domain.
  • 翻译后修饰Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.
    Stimulated by phosphorylation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cAMP response element modulator antibody
    • cAMP responsive element modulator antibody
    • cAMP-responsive element modulator antibody
    • CREM antibody
    • CREM-2 antibody
    • CREM_HUMAN antibody
    • hCREM 2 antibody
    • hCREM-2 antibody
    • hCREM2 antibody
    • ICER antibody
    • Inducible cAMP early repressor antibody
    • Inducible cAMP early repressor ICER antibody
    • MGC111110 antibody
    • MGC17881 antibody
    • MGC41893 antibody
    see all

Anti-CREM antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab54625 CREM antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

  • Overlay histogram showing HeLa cells stained with ab54625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54625, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-CREM antibody (ab54625)参考文献

This product has been referenced in:
  • Cardone J  et al. Complement regulator CD46 temporally regulates cytokine production by conventional and unconventional T cells. Nat Immunol 11:862-71 (2010). WB ; Human . Read more (PubMed: 20694009) »

See 1 Publication for this product

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