Anti-CREB3L2抗体(ab102989)

概述

  • 产品名称
    Anti-CREB3L2抗体
    参阅全部 CREB3L2 一抗
  • 描述
    兔多克隆抗体to CREB3L2
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 491-520 of Human CREB3L2

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab102989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 57 kDa.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Transcriptional activator which is processed and activated during endoplasmic reticulum stress late phase. Binds to the cAMP response element (CRE) and activates transcription through CRE. Regulates the transcription of unfolded protein response target genes, preventing accumulation of unfolded proteins in damaged neurons. Also regulates the expression of SEC23A, accelerating protein trafficking from the ER to the Golgi thereby playing a key role in chondrocyte differentiation and formation of epiphyseal cartilage.
  • 组织特异性
    Widely expressed with highest levels in placenta, lung, spleen and intestine, and lowest levels in heart, brain, skeletal muscle, thymus, colon and leukocytes. In fetal tissues, the weakest expression is detected in brain and heart.
  • 疾病相关
    A chromosomal aberration involving CREB3L2 is found in low grade fibromyxoid sarcoma (LGFMS). Translocation t(7;16)(q33;p11) with FUS.
  • 序列相似性
    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • 翻译后修饰
    Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (PS1 and PS2).
  • 细胞定位
    Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B ZIB transcription factor antibody
    • Basic transcription factor 2 antibody
    • BBF2 human homolog on chromosome 7 antibody
    • BBF2H7 antibody
    • BZIB transcription factor antibody
    • cAMP responsive element binding protein 3 like 2 antibody
    • cAMP-responsive element-binding protein 3-like protein 2 antibody
    • CR3L2_HUMAN antibody
    • Creb3l2 antibody
    • Cyclic AMP-responsive element-binding protein 3-like protein 2 antibody
    • FUS/BBF2H7 protein antibody
    • MGC131709 antibody
    • MGC71006 antibody
    • Processed cyclic AMP-responsive element-binding protein 3-like protein 2 antibody
    • TCAG_1951439 antibody
    see all

图片

  • Anti-CREB3L2 antibody (ab102989) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size : 57 kDa
  • ab102989 staining CREB3L2 in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 1.5% serum for 1 hour at 25°C and then incubated with ab102989 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an undiluted biotin conjugated goat polyclonal.

    See Abreview

  • Immunofluorescence analysis of U2-OS, staining CREB3L2 with ab102989.

    Cells were fixed with formaldehyde, permeabilized with 0.5% NP-40 in PBS and blocked with 3% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 3% BSA in PBS) for 12 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

文献

ab102989 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Oct 31 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% NP40/PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 25 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (primary vascular smooth muscle cells)
Specification
primary vascular smooth muscle cells
Fixative
Methanol/Acetone
Permeabilization
Yes - 0.5% NP-40
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

提交于 Mar 14 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Vector Labs Unmasking Solution H-3300
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

提交于 Mar 14 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (SAOS-2, U2OS, HEK 293T, ATDC5)
Loading amount
15 µg
Specification
SAOS-2, U2OS, HEK 293T, ATDC5
Treatment
0.02 ug/ml tunicamycin for 72h
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

提交于 Dec 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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