Anti-CREB3L1抗体(ab112021)

概述

  • 产品名称Anti-CREB3L1抗体
    参阅全部 CREB3L1 一抗
  • 描述
    山羊多克隆抗体to CREB3L1
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    C-DFSHSKEWFHDRD

    , corresponding to C terminal amino acids 497-509 of Human CREB3L1.

  • 阳性对照
    • Human Liver and Human Skeletal Muscle tissue; A549 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab112021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 3.75 µg/ml.
ELISA 1/32000.

靶标

  • 功能Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Specifically involved in ER-stress response in astrocytes in the central nervous system (By similartity). May play a role in gliosis. In vitro, binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE.
  • 组织特异性Ubiquitously expressed with high levels in pancreas and prostate. Expressed at relatively lower levels in brain.
  • 序列相似性Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • 翻译后修饰Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is performed sequentially by site-1 and site-2 proteases (PS1 and PS2) and is triggered by translocation to the Golgi apparatus.
  • 细胞定位Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BBF-2 homolog antibody
    • CAMP responsive element binding protein 3 like 1 antibody
    • cAMP-responsive element-binding protein 3-like protein 1 antibody
    • CR3L1_HUMAN antibody
    • Creb3l1 antibody
    • Cyclic AMP responsive element binding protein 3 like protein 1 antibody
    • OASIS antibody
    • Old astrocyte specifically-induced substance antibody
    • Processed cyclic AMP-responsive element-binding protein 3-like protein 1 antibody
    see all

Anti-CREB3L1 antibody 图像

  • Immunohistochemical analysis of CREB3L1 expression in formalin-fixed, paraffin-embedded Human liver using ab112021 at 3.75 µg/ml.
  • Immunohistochemical analysis of CREB3L1 expression in formalin-fixed, paraffin-embedded Human skeletal muscle using ab112021 at 3.75 µg/ml.
  • Anti-CREB3L1 antibody (ab112021) at 3 µg/ml + A549 cell lysate

    Predicted band size : 57 kDa

Anti-CREB3L1 antibody (ab112021)参考文献

ab112021 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (SAOS-2, U2OS, HEK 293T, ATDC5)
Loading amount 15 µg
Specification SAOS-2, U2OS, HEK 293T, ATDC5
Treatment 0.02 ug/ml tunicamycin for 72h
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

提交于 Dec 15 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human vascular smooth muscle cells)
Specification human vascular smooth muscle cells
Fixative Methanol/Acetone
Permeabilization Yes - 0.5% NP-40
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

提交于 Nov 30 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Vector Labs Unmasking Solution H-3300
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
Username

Leilani Beltran

Verified customer

提交于 Nov 30 2011

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab33051 with the order number. This is currently out of stock, but you can expect ...

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Thank you for your reply. You can see the WB data on our online datasheet. http://www.abcam.com/CREB3L1-antibody-ab112021.pdf Please let me know if the protocol suggestions do not improve your results.  I look forward to your reply.

Thank you for your reply and for sending your protocol and images. There are a few adjustments to the protocol that you may want to try. For nuclear proteins, these can sometimes be difficult to extract.  We usually recommend the addition of SDS fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"