概述

  • 产品名称Anti-CREB + CREM抗体
  • 描述
    兔多克隆抗体to CREB + CREM
  • 特异性ab5803 detects both the phosphorylated and non-phosphorylated forms of cyclic-AMP response element binding protein (CREB) from rat cells.
  • 经测试应用适用于: WB, EMSA, ICC/IF, ChIP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
    预测可用于: Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human CREB aa 123-136.
    Sequence:

    KRREILSRRPSYRK


    (Peptide available as ab5860)

  • 阳性对照
    • GH4 cell extract.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab5803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Can be blocked with CREB + CREM peptide (ab5860).
EMSA Use at an assay dependent concentration. PubMed: 16901903
ICC/IF 1/50 - 1/500.
ChIP Use at an assay dependent concentration.

1-3 uL

IHC-P 1/20 - 1/200.

Anti-CREB + CREM antibody 图像

  • Immunofluorescent analysis of CREB (green) showing staining in the nucleus of SK-N-MC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB polyclonal antibody (ab5803) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

  • Immunohistochemistry analysis of CREB showing staining in the nucleus of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CREB polyclonal antibody (ab5803) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry analysis of CREB showing staining in the nucleus of paraffin-embedded Human glioma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CREB polyclonal antibody (ab5803) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry analysis of CREB showing staining in the nucleus of paraffin-embedded Human lung adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CREB polyclonal antibody (ab5803) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunofluorescent analysis of CREB (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB polyclonal antibody (ab5803) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.



  • Predicted band size : 43 kDa

    Shows a Western blot of CREB on GH4 cell extract using ab5803.

  • All lanes : Anti-CREB + CREM antibody (ab5803) at 1/500 dilution

    Lane 1 : hippocampal lysate
    Lane 2 : hippocampal lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    Donkey Anti-Rabbit IR800-linked conjugated to IRDye 800CW at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 55 kDa (possible non-specific binding).

    Exposure time : 5 minutes

    This image is courtesy of Richard D'Mello, Kings College, London

Anti-CREB + CREM antibody (ab5803)参考文献

This product has been referenced in:
  • Singh SK  et al. GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy. Nat Commun 4:1830 (2013). Rat . Read more (PubMed: 23652018) »
  • Melas PA  et al. Allele-specific programming of Npy and epigenetic effects of physical activity in a genetic model of depression. Transl Psychiatry 3:e255 (2013). Read more (PubMed: 23652932) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step PBS-1% ovalbumin as blocking agent for 20 minute(s) · Concentration: 1.0% · Temperature: 22°C
Sample Salmo salar Cell (testicular)
Specification testicular
Permeabilization Yes - methyl methacrylate/Butyl methacrylate
Fixative glutaraldehyde-formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 25 2015

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing
Sample Cow Tissue lysate - whole (Subcutaneous Adipose)
Specification Subcutaneous Adipose
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Nov 08 2013

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which gu...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Hippocampus lysate)
Loading amount 40 µg
Specification Hippocampus lysate
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Richard D'Mello

Verified customer

提交于 Sep 27 2010

Le pido que nos disculpe por la falta en su orden. He hecho una nueva orden cual le sera enviada lo mas pronto posible. Porfavor refiera al numero de orden 68450 si necesita mas información sobre el estado de su orden. Otra vez, porfavor disculpe la fa...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"