使用敲除细胞株进行验证RabMAb

Anti-CREB抗体[48H2] (ab49762)

概述

  • 产品名称Anti-CREB抗体[48H2]
    参阅全部 CREB 一抗
  • 描述
    兔单克隆抗体[48H2] to CREB
  • 经测试应用适用于: WB, IP, IHC-Fr, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Monkey
  • 免疫原

    CREB full length fusion protein.

  • 阳性对照
    • SK-N-MC, NIH-3T3, and COS cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab49762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 37 kDa.
IP 1/250.
IHC-Fr 1/400.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/1600.
Flow Cyt 1/400. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
  • 疾病相关Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
  • 序列相似性Belongs to the bZIP family.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • 翻译后修饰Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Active transcription factor CREB antibody
    • cAMP response element binding protein 1 antibody
    • cAMP response element binding protein antibody
    • cAMP responsive element binding protein 1 antibody
    • cAMP-responsive element-binding protein 1 antibody
    • CREB antibody
    • CREB-1 antibody
    • CREB1 antibody
    • CREB1_HUMAN antibody
    • Cyclic AMP-responsive element-binding protein 1 antibody
    • MGC9284 antibody
    • OTTHUMP00000163864 antibody
    • OTTHUMP00000163865 antibody
    • OTTHUMP00000206660 antibody
    • OTTHUMP00000206662 antibody
    • OTTHUMP00000206667 antibody
    • Transactivator protein antibody
    see all

Anti-CREB antibody [48H2] 图像



  • Predicted band size : 37 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CREB knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab49762 observed at 44 kDa. Red - loading control, ab18058, observed at 124 kDa.
    ab49762 was shown to recognize CREB when CREB knockout samples were used, along with additional cross-reactive bands. Wild-type and CREB knockout samples were subjected to SDS-PAGE. ab49762 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • ab49762 staining CREB in rat brain cortex and cerebellum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed using a zinc fixative and blocked with biotin for 16 minutes at 37°C. Samples were incubated with primary antibody (9 ug/mL) for 1 hour at 37°C. A biotinylated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

Anti-CREB antibody [48H2] (ab49762)参考文献

ab49762 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain Cortex and cerebellum)
Permeabilization No
Specification Brain Cortex and cerebellum
Blocking step Ventana Endogenous Biotin Blocking Kit (cat.# 760-050) as blocking agent for 16 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative BD Pharmigen IHC Zinc Fixative (cat.# 51-7538KZ)
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提交于 Feb 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"