概述

  • 产品名称
    Creatinine Assay试剂盒
    参阅全部 Creatinine 试剂盒
  • 检测方法
    Colorimetric/Fluorometric
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • 检测类型
    Quantitative
  • 检测时间
    1h 00m
  • 产品概述

    Creatinine Assay Kit (ab65340) provides an accurate, convenient measure of creatine concentration in biological fluids such as serum, urine or CSF. In the assay, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 538/587 nm). Unlike picric acid assays, this kit is suitable for serum/plasma creatinine determinations, as well as for urine and other biological samples.


    For deproteinization of samples:  10kda method works the beter for deproteinization when preparing samples as opposed to PCA method. It’s possible that the chemicals used negatively affects the enzyme components of the assay kit.



    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Creatinine is a breakdown product of creatine phosphate. Creatinine is produced and excreted at a constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function. Blood creatinine levels increase only in cases of significant (>75%) damage to nephrons. Creatinine clearance is frequently used as a means of standardizing excretion of other compounds such as isoprostanes.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab65340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

图片

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Creatinine measured in mouse and human serum plotted against RFU. Samples were diluted 5-10 fold.

  • Creatinine measured in mouse and human urine plotted against RFU. Samples were diluted 400-800 fold.

  • Creatinine levels in filtered human urine was measured in the presence or absence of creatininase (background signal subtracted.

实验方案

文献

This product has been referenced in:
  • Wolley MJ  et al. In Primary Aldosteronism, Mineralocorticoids Influence Exosomal Sodium-Chloride Cotransporter Abundance. J Am Soc Nephrol 28:56-63 (2017). Functional Studies . Read more (PubMed: 27381844) »
  • Gai Z  et al. Farnesoid X Receptor Protects against Kidney Injury in Uninephrectomized Obese Mice. J Biol Chem 291:2397-411 (2016). Read more (PubMed: 26655953) »

See all 17 Publications for this product

客户评价及客户问答

Filter by Ratings

Creatinine in porcine urine samples

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Tested the kit in porcine urine samples and after deproteinisation, the kit worked. We used the colourimetric assay as per protocol in the booklet.
We used the deproteinisation protocol with PCA and KOH as provided in the booklet that accompanies the kit.
Had to dilute the deproteinised samples 50x to bring samples into the expected detectable range.
Username

Abcam user community

Verified customer

提交于 Jul 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册