Anti-Creatine Kinase MM抗体(ab83441)

概述

  • 产品名称Anti-Creatine Kinase MM抗体
    参阅全部 Creatine Kinase MM 一抗
  • 描述
    兔多克隆抗体to Creatine Kinase MM
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 71-120 (VGCVAGDEESYEVFKELFDPIISDRHGGYKPTDKHKTDLNHENLKGGDD L) of Human CKM (NP_001815).

  • 阳性对照
    • Human fetal muscle lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre in peptide based assay is 1/1562500.

靶标

Anti-Creatine Kinase MM antibody 图像

  • Anti-Creatine Kinase MM antibody (ab83441) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1/50000 - 1/100000 as secondary antibody.

    Predicted band size : 43 kDa

Anti-Creatine Kinase MM antibody (ab83441)参考文献

ab83441 has not yet been referenced specifically in any publications.

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I'm glad the information was of help to you.

I can now confirm to you that the antibody ab38178 against CKMM is not likely to be able to detect the horse protein as the immunogen used to raise itonly shares 76% homology. I would therefore sug...

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Thank you for confirming those details. I'm sorry it has taken me some time to get back to you, this has not been a trivial task but I hope I now have the information you were hoping for.

CKMM

For this target, I am still waiting on ...

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Thank you for contacting us.

I have checked the following alignments with the following homology results:
CK BB: human-mouse: 97%
CK MM: human-mouse: 97%
CK MB: human-mouse: 97% - there is no separate sequence for MB as the pr...

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