概述

  • 产品名称Anti-Creatine Kinase MM抗体
    参阅全部 Creatine Kinase MM 一抗
  • 描述
    小鼠单克隆抗体to Creatine Kinase MM
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: TCPSNLGTGL RGGVHVKLAH LSKHPKFEEI LTRLRLQKRG TGGVDTAAVG SVFDVSNADR LGSSEVEQVQ LVVDGVKLMV EMEKKLEKGQ SIDDMIPAQK , corresponding to amino acids 282-382 of Human Creatine Kinase MM

性能

应用

Our Abpromise guarantee covers the use of ab54637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • 序列相似性Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CKM antibody
    • CKMM antibody
    • Creatine kinase M antibody
    • Creatine kinase M chain antibody
    • Creatine kinase M type antibody
    • Creatine kinase M-type antibody
    • Creatine kinase muscle antibody
    • Creatine kinase, muscle type antibody
    • KCRM_HUMAN antibody
    • M-CK antibody
    • MCK antibody
    • Muscle creatine kinase antibody
    see all

Anti-Creatine Kinase MM antibody 图像



  • Predicted band size : 43 kDa
    Creatine Kinase MM antibody (ab54637) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.
  • ab54637 at 10 µg/ml staining Creatine Kinase MM in human Hela cells by Immunocytochemistry / Immunofluorescence.

Anti-Creatine Kinase MM antibody (ab54637)参考文献

This product has been referenced in:
  • Zhang GJ  et al. An integrated chip for rapid, sensitive, and multiplexed detection of cardiac biomarkers from fingerprick blood. Biosens Bioelectron 28:459-63 (2011). Read more (PubMed: 21807497) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Tissue lysate - whole (skeletal muscle)
Specification skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Rafael Montes Nieto

Verified customer

提交于 Oct 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"