Anti-Creatine Kinase MM抗体[3E5BA5] (ab110319)
Key features and details
- Mouse monoclonal [3E5BA5] to Creatine Kinase MM
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG2a
概述
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产品名称
Anti-Creatine Kinase MM抗体[3E5BA5]
参阅全部 Creatine Kinase MM 一抗 -
描述
小鼠单克隆抗体[3E5BA5] to Creatine Kinase MM -
宿主
Mouse -
经测试应用
适用于: WBmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Human -
免疫原
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human heart homogenate. WB: Mouse and Human skeletal muscle tissue lysates.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
ab110319 was produced in vitro using hybridomas grown in serum-free medium. Purity: >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
3E5BA5 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110319于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. |
靶标
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功能
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
序列相似性
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 1158 Human
- Entrez Gene: 12715 Mouse
- Omim: 123310 Human
- SwissProt: P06732 Human
- SwissProt: P07310 Mouse
- Unigene: 334347 Human
- Unigene: 2375 Mouse
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别名
- CKM antibody
- CKMM antibody
- Creatine kinase M antibody
see all
图片
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All lanes : Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319) at 1/50 dilution
Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Mouse placenta tissue lysate
Lane 4 : Human skeletal muscle tissue lysate
Lane 5 : Human spleen tissue lysate
Lane 6 : Human placenta tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse lgG (H+L) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab129002 was used as a loading control.
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Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319) at 1 µg/ml + Human heart homogenate at 20 µg
Predicted band size: 43 kDa
数据表及文件
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Datasheet download
文献 (0)
ab110319 尚未被引用在任何文献中。