使用敲除细胞株进行验证RabMAb

Anti-Creatine kinase B type抗体[EPR3927] (ab92452)

概述

  • 产品名称Anti-Creatine kinase B type抗体[EPR3927]
    参阅全部 Creatine kinase B type 一抗
  • 描述
    兔单克隆抗体[EPR3927] to Creatine kinase B type
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of Human Creatine Kinase B type.

  • 阳性对照
    • SHSY-5Y, HeLa, Y-79 and SW840 cell lysate. Human glioma tissue.
  • 常规说���

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

应用

Our Abpromise guarantee covers the use of ab92452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/500.

Antigen retrieval and the use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining.

Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/500.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
    • 序列相似性Belongs to the ATP:guanido phosphotransferase family.
      Contains 1 phosphagen kinase C-terminal domain.
      Contains 1 phosphagen kinase N-terminal domain.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • B CK antibody
      • B-CK antibody
      • BB-CK antibody
      • BCK antibody
      • Brain creatine kinase antibody
      • Ckb antibody
      • CKBB antibody
      • Creatine kinase B antibody
      • Creatine kinase B chain antibody
      • Creatine kinase B type antibody
      • Creatine kinase B-type antibody
      • Creatine Kinase BB Isoenzyme antibody
      • Creatine kinase brain antibody
      • Creatine phosphokinase BB antibody
      • KCRB_HUMAN antibody
      see all

    Anti-Creatine kinase B type antibody [EPR3927] 图像



    • Predicted band size : 43 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Creatine kinase B knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: SH-SY5Y cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab92452 observed at 45 kDa. Red - loading control, ab18058, observed at 2X kDa.
      ab92452 was shown to specifically react with Creatine kinase B when ProteinX knockout samples were used. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab92452 and ab18058 (loading control to vinculin) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Creatine kinase B type antibody [EPR3927] (ab92452) at 1/10000 dilution

      Lane 1 : SHSY-5Y cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Y-79 cell lysate
      Lane 4 : SW840 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 43 kDa
    • Paraffin embedded Human glioma tissue labelled with ab92452 at 1/100 dilution.
    • Immunofluorescence analysis of HeLa cells labelled with ab92452 at 1/100 dilution.
    • Overlay histogram showing HeLa cells stained with ab92452 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92452, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Creatine kinase B type antibody [EPR3927] (ab92452)参考文献

    ab92452 has not yet been referenced specifically in any publications.

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