Anti-Creatine kinase B type抗体(ab38211)

概述

  • 产品名称Anti-Creatine kinase B type抗体
    参阅全部 Creatine kinase B type 一抗
  • 描述
    兔多克隆抗体to Creatine kinase B type
  • 特异性This antibody is specific for Creatine kinase BB.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide selected within aa 200~300 of human Creatine Kinase BB.

  • 阳性对照
    • Y79 cell lysate and mouse colon tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab38211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

靶标

  • 功能Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • 序列相似性Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine phosphokinase BB antibody
    • KCRB_HUMAN antibody
    see all

Anti-Creatine kinase B type antibody 图像

  • All lanes : Anti-Creatine kinase B type antibody (ab38211) at 1/100 dilution

    Lane 1 : Y79 cell lysate
    Lane 2 : Mouse colon tissue lysate


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • Anti-Creatine kinase B type antibody (ab38211) at 1/250 dilution + Tissue lysate prepared from human brain tissue at 10 µg

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/7500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa


    Exposure time : 1 minute

    This image is a courtesy of Anonymous Abreview

    See Abreview

Anti-Creatine kinase B type antibody (ab38211)参考文献

This product has been referenced in:
  • Mochel F  et al. Early alterations of brain cellular energy homeostasis in huntington disease models. J Biol Chem 287:1361-70 (2012). WB ; Mouse . Read more (PubMed: 22123819) »
  • Zhang SF  et al. Impaired brain creatine kinase activity in Huntington's disease. Neurodegener Dis 8:194-201 (2011). Mouse . Read more (PubMed: 21124007) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us.

I have checked the following alignments with the following homology results:
CK BB: human-mouse: 97%
CK MM: human-mouse: 97%
CK MB: human-mouse: 97% - there is no separate sequence for MB as the pr...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Brain)
Loading amount 10 µg
Specification Brain
Treatment homogenisation in guanidine thiocyanate with subsequent ultracentrifugation over caesium chloride and dialysis
Gel Running Conditions Reduced Denaturing (4-20%, 1mm)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (brain)
Loading amount 5 µg
Specification brain
Gel Running Conditions Reduced Denaturing (4-12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 14 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"