使用敲除细胞株进行验证

Anti-Creatine kinase B type抗体[3A6] (ab71944)

概述

  • 产品名称Anti-Creatine kinase B type抗体[3A6]
    参阅全部 Creatine kinase B type 一抗
  • 描述
    小鼠单克隆抗体[3A6] to Creatine kinase B type
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Human Creatine kinase B type.

  • 阳性对照
    • Human Creatine Kinase MB protein. Recombinant Creatine Kinase BB protein. Recombinant Creatine Kinase MB protein.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab71944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).

靶标

  • 功能Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • 序列相似性Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine phosphokinase BB antibody
    • KCRB_HUMAN antibody
    see all

Anti-Creatine kinase B type antibody [3A6] 图像



  • Predicted band size : 43 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Creatine kinase B type knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: SHSY5Y cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab71944 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab71944 was shown to specifically react with Creatine kinase B type when Creatine kinase B type knockout samples were used. Wild-type and Creatine kinase B type knockout samples were subjected to SDS-PAGE. ab71944 and ab181602 (loading control to GAPDH) were diluted at 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Creatine kinase B type antibody [3A6] (ab71944) at 1/2000 dilution

    Lane 1 : Recombinant CK-MM protein isolated from E. coli
    Lane 2 : Recombinant CK-BB protein isolated from E. coli
    Lane 3 : Recombinant CK-MB protein isolated from E. coli
    Lane 4 : Human CK-MB protein


    Predicted band size : 43 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)

Anti-Creatine kinase B type antibody [3A6] (ab71944)参考文献

ab71944 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab71944.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"